Abstract
Detection of Brucella in Tissues and in Formalin-Fixed Paraffin-Embedded (FFPE) Specimens Using PCR
Highlights
Brucellosis is an important zoonosis causing reproductive failures and significant economic losses in livestock, (Boschiroli et al, 2001; Al Dahouk et al, 2005; Bricker and Ewalt, 2005; Xavier et al, 2009a)
Typing of Brucella isolates recovered in this study revealed Brucella melitensis biovar 3 (Table 4)
Depletion of lymphocytes was seen in the white pulp of spleen and the cortex of lymph nodes, associated with presence of fatty infiltration in some cases
Summary
Brucellosis is an important zoonosis causing reproductive failures and significant economic losses in livestock, (Boschiroli et al, 2001; Al Dahouk et al, 2005; Bricker and Ewalt, 2005; Xavier et al, 2009a). The disease is caused by bacteria of the genus Brucella. Diagnosis of brucellosis depends on detection of Brucella spp., demonstration of specific antibody or cell-mediated immune responses as well as histopathological investigation. Because of the difficulties and limitations of the culture technique (Alton et al, 1988; Seleem et al, 2010) and the complexity of serological diagnosis that requires the application of battery of sero- tests (OIE Terrestrial Manual, 2012), the use of molecular techniques for detection of Brucella infection especially from tissue specimens was attempted by several researchers
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