Abstract
The main object of this study is to detect the Brucella spp. in the marketable milk sold in Alexanderia city by using different methods such as MRT (milk ring test), ELISA, direct culture and PCR. A total of 170 milk samples were purchased as 70 cow’s milk samples and 100 buffalo’s milk samples. The obtained results indicated that the incidence of Brucella antibodies in milk samples were estimated by MRT in 16 samples (7 cow’s milk and 9 buffalo’s milk) out of the 170 milk samples ; and by ELISA in 35 samples (19 cow’s milk and 16 buffalo’s milk) out of the 170 milk samples. Moreover, Brucella spp. were detected in 4.3 % of the cow’s milk samples and 5 % of the buffalo’s milk samples by direct culture. Also the incidence of Brucella spp. gene were detected in 14 samples (8.2%) out of the 170 milk samples as 6 (8.6 %) for cow’s milk and 8 (8%) for buffalo’s milk samples by using PCR. In conclusion, PCR proved to be more suitable tools for Brucella detection than the culture techniques. A combination between molecular techniques and conventional techniques found to be a good reliable policy for controlling the disease. Achieved results set a warning for public health hazard due to habit of drinking of fresh raw milk
Highlights
Brucellosis, known as“undulant fever”, “Mediterranean fever” or “Malta fever” is a highly contagious bacterial zoonotic disease that affect millions of people worldwide and a wide variety of farm animals (Mohsen, 2000; Bricker, 2002) and still remains a significant public health and economic problem in many developing countries (Hassan and Samaha, 2008)
Brucellosis is common in rural areas because farmers live in close contact with their animals and often consume fresh unpasteurized dairy products
Higher and lower results for detection of Brucellaein raw milk by using milk ring test (MRT) and ELISA were recorded by many researchers [Farag (1998), Hamdy and Amin (2002), Abdalla and Hamid (2012), Ibrahim et al (2012) and Abo-shama, (2013)]
Summary
Brucellosis, known as“undulant fever”, “Mediterranean fever” or “Malta fever” is a highly contagious bacterial zoonotic disease that affect millions of people worldwide and a wide variety of farm animals (Mohsen, 2000; Bricker, 2002) and still remains a significant public health and economic problem in many developing countries (Hassan and Samaha, 2008). The methods for the diagnosis of brucellosis firstly by serological tests via detection of antibodies specific for Brucella infection (Refai, 2003). Serological methods are not conclusive, because of the absence of the detectable level of antibodies by the infected animals.
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