Abstract
The ability to determine the sex of embryos prior to transfer to recipients has commercial application in the embryo transfer industry. A number of methods of sex determination have been attempted; however, none of these has been commercially successful to date. The identification of repetitive, male-specific bovine chromosomal DNA fragments enables the use of DNA-probe technology in determining the sex of preimplantation embryos from a small embryonic sample. The identification and isolation of three repetitive, male-specific bovine chromosomal DNA fragments, and the application of these probes in a sensitive and highly accurate embryo sexing assay are reported.
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