Abstract
DNA extracted from serum samples collected from 114 apparently healthy and 71 clini- cally sick pet dogs of different age, sex, breed and place was used as templet for detection of Bordetella infection through genus specific PCR (gPCR) targeting alc gene and species specific PCR (sPCR) targeting fim gene. Bordetella DNA could be detected in serum of 7 apparently healthy and 7 sick dogs both with gPCR and sPCR. Clinical status (p= 0.35), age (p= 0.31) and sex (p= 0.72) had no significant association with detection of Bordetella DNA in serum samples. Of the 14 samples positive 12 (85.7%) were from Kerala and two (14.3%) from Manipur dogs. Sequencing of the gel purified gPCR product targeting alc gene revealed 95% homology with alc gene sequence of several species of Bordetella and sequencing of the gel purified sPCR product targeting fim gene revealed 100% identity with Bordetella bronchiseptica fim gene. The study suggested that B. bronchiseptica might be the most common bordetellae affecting pet dogs irrespective of clinical illness. The study conclud- ed that PCR with serum extracted DNA template can be an option for rapid detection of Bordetella infection in pet dogs.
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