Abstract
A reverse transcription-polymerase chain reaction (RT-PCR) assay has been developed for the detection of strain NJ2 of blueberry scorch carlavirus (BBScV), which causes an economically important disease of blueberry. Four extraction methods were compared for their ability to yield nucleic acid from blueberry tissue that could be amplified by specific RT-PCR primers. Two extraction methods were found suitable when they were followed by an Elutip-r column purification step. These procedures were successful with blueberry blossoms and leaves. RT-PCR was shown to be considerably more sensitive than spot hybridization using a 32 P-labeled DNA probe. These purification systems enable use of nucleic acid-based RNA virus detection from blueberry.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
