Abstract
Ethyl acetate extracts of fermentation cultures of five local Streptomyces isolates SRY-3, SRY-25, 5b, 10, NS-38 were identified as inhibitors of plasmacytoma cell line. Concentration at 0.016 µg/ml of ethyl acetate extract of (SRY-3,SRY-25,5b,10,NS-38) inhibited (46%,40%,21%,34%,34%) of mouse plasmacytoma cells respectively, where as 0.5 µg/ml inhibited (58%, 55%, 35%, 33%, 36%) of plasmacytoma cells .Quantities analysis of crude extracts by using HPLC on the basis of their retention times showed that the values were (4.3,4.4,4.86,4.83&4.84) min. for SRY-3, SRY-25, 5b, 10,NS-38 respectively, while the retention time of standard antitumor compounds was (4.26) min. This suggests that unknown compound of SRY-3 extract contain bleomycin-like compound. Addition of standard bleomycin to crude extract of SRY-3 increased milliabsorbans unit (M.A.U.) from 0.49 to 1.4. The concentration of bleomycin-like antitumor in fermentation broth of SRY-3 isolate was 3.441 µg/ml.The five Streptomyces isolates have moderate activity against gram-positive and gram-negative bacteria, while standard BLM have no activity.
Highlights
Ethyl acetate extracts of fermentation cultures of five local Streptomyces isolates SRY-3, SRY-25, 5b, 10, NS-38 were identified as inhibitors of plasmacytoma cell line
Streptomycetes have considerable medical, biological and commercial importance [1], since they produce over 60% of known antibiotics and many other substances with valuable clinical and other applications [2]. Various antitumor compounds are produced by streptomycetes, which have activities against different types of tumor cells [3,4,5]
Ninety-six well tissue culture plates were used for this test to ensure the toxic effect of crude extract of fermentation culture broth of SRY-3, SRY-25, 5b, 10, NS38 on plasmacytoma SU 99 cell line
Summary
Microorganisms: Local isolates of Streptomyces SRY-3, SRY-25, 5b, 10, NS-38 were used throughout this work. Ninety-six well tissue culture plates were used for this test to ensure the toxic effect of crude extract of fermentation culture broth of SRY-3, SRY-25, 5b, 10, NS38 on plasmacytoma SU 99 cell line. The wells of the late four lines in the same plate were filled with 0.2ml tissue culture medium only considered as a control. A 0.15ml cell suspension of plasmacytoma cell culture (104 cells per ml) was added to Analysis of bleomycin by HPLC: The quantitative and qualitative assays of bleomycin were performed by high performance liquid chromatography (HPLC) the eluents were injected into the HPLC: column: shimi – pock Antimicrobial activity: Block assay method was used to determine the antimicrobial activity of Streptomyces isolates grown on scarped and eluted with methanol and dried for further investigation. Antibiotic Susceptibility: Antibiotic resistances of Streptomyces isolates were examined by using antibiotic sensitivity discs [19]
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