Abstract
Rice blast disease caused by Pyricularia oryzae is a major disease in Bangladesh. In the search of resistant source against P. oryzae, resistant gene (R-gene) was assessed by (i) host-pathogen interaction and (ii) PCR-based screening at the laboratory of Bangladesh Rice Research Institute. One hundred and forty four plant materials were classified into 10 cultivar groups (CG) based on their reaction patterns to four distinct blast isolates (SDBI; standard differential blast isolate) H-11-64, H-1-8, H-1-1 and H-11-67. The reaction patterns of plant materials to these four standard blast isolates indicated presence of Pish, Pi9, Pita2 and Pita genes and their combination in the genetic background of tested plant materials. Gene specific primer 195R-1/195F-1, Pita440 (YL153)/Pita440 (YL154), OSM89 and AOL45/AOL48 were used to identify and confirm the presence of Pi9, Pita, Pita2 and Pish genes in plant materials, respectively through PCR-based assay. Plant materials, H13, H23, H25, H35, H47, H49, H136, H138 harbored all three genes, Pita, Pita2 and Pish in their genetic background. The Pi9 gene together with Pita2 was detected in local rice cultivar H100 and H129, which were confirmed by DNA analysis in PCR assay. These materials could be used in gene pyramiding in promising high yielding cultivar for durable blast resistance.
Highlights
Rice (Oryza sativa L.) is the staple food for about half of the world’s population
Pyramiding of resistant gene (R-gene) is needed for development of durable resistant rice cultivars against blast pathogen
Four standard differential blast isolates (SDBIs) viz. H-11-64, H-1-8, H-1-1(incompatible to Pita -2 gene) and H-11-67 were collected from Hossain [9] to identify those resistant genes in rice land races through pathogenicity test
Summary
Rice (Oryza sativa L.) is the staple food for about half of the world’s population. Bangladeshi people take Rice (Oryza sativa L.) as a major food which is central to the economy of the country accounting for about 20% of GDP (gross domestic product) [1, 2] and as more than 90% of all rice is produced in Asia it is called an Asian crop. Among various factors responsible for low yield of rice, blast caused by P. oryzae plays an important role which causes yield loss of 11-15% worldwide annually [3]. Under climatic changes such as lacking in water, many rice diseases blast disease of rice may increase [4, 5]. Agricultural land in Bangladesh is declining but population is raising day by day, means that mounting of rice yield must be explored from different approaches. In such situation resistant cultivars is one of the potential ways to control blast pathogen. Pyramiding of R-gene is needed for development of durable resistant rice cultivars against blast pathogen
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