Abstract
: Bioflim is a mode of survival for various microbe by which they form aggregates during unfavourable conditions. is a major cause of nosocomial and community acquired infections.A total of 150 isolates were screened for biofilm production by Congored method and Tube method following standard guidelines.Of the 150 isolates, 85(56.6%) were MSSA and 65(43.4%) were MRSA. On Congored agar, 63 isolates showed black colonies with dry crystalline consistency indicating biofilm production. Out of 63 isolates 62% of isolates were MRSA and 38% of isolates were MSSA. Biofilm by tube method, 84isolates showed biofilm production. MSSA were 48.2% and MRSA were 81.53%.MRSA is the significant biofilm producer when compare to MSSA, Congored method is less accurate when compare to tube method as screening test for the detection of biofilm.
Highlights
Staphylococcus.aureus is a commensal bacteria on the human skin and mucosa and a prominent human pathogen that can cause healthcare associated infections and community associated skin and soft tissue infections. 1 Staphylococcus has an ability of acquiring drug resistance and bioflim production in indwelling medical devices making them an important pathogen. 1Staphylococcal bioflim can develop on various structures such as prosthetic joints, prosthetic heart valves, catheters, contact lenses, cardiac pacemakers and cerebrospinal fluid shunts. 2 80% of Nosocomial infections are due to bioflim production
A total of 150 isolates were detected for bioflim production by Congored method and Tube method
A total of 150 isolates were tested for biofilm production by congored method
Summary
Staphylococcus.aureus is a commensal bacteria on the human skin and mucosa and a prominent human pathogen that can cause healthcare associated infections and community associated skin and soft tissue infections. 1 Staphylococcus has an ability of acquiring drug resistance and bioflim production in indwelling medical devices making them an important pathogen. 1Staphylococcal bioflim can develop on various structures such as prosthetic joints, prosthetic heart valves, catheters, contact lenses, cardiac pacemakers and cerebrospinal fluid shunts. 2 80% of Nosocomial infections are due to bioflim production. Staphylococcus.aureus is a commensal bacteria on the human skin and mucosa and a prominent human pathogen that can cause healthcare associated infections and community associated skin and soft tissue infections. 2 80% of Nosocomial infections are due to bioflim production. Bioflim productions by Staphylococcus.aureus begins with adhesion of bacteria to inert /biotic surface with help of adhesion factor microbial surface components recognizing adhesive matrix molecules (MSCRAMMS). Staphylococcus aureus is a major cause of nosocomial and community acquired infections. Materials and Methods: A total of 150 Staphylococcus isolates were screened for biofilm production by Congored method and Tube method following standard guidelines. Conclusion: MRSA is the significant biofilm producer when compare to MSSA, Congored method is less accurate when compare to tube method as screening test for the detection of biofilm
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