Detection of benzodiazepines and other psychotropic drugs in human hair by GC/MS

Abstract

For the detection of psychotropic drugs in human hair we collected hair obtained from 21 corpses that died from an overdose of legal or illicit drugs. These persons were known to have taken psychotropic drugs prior to their death as determined by post-mortem toxicological analysis in blood. After washing, cutting hair into segments of 3 cm, pulverization, enzymatic hydrolysis with β-glucuronidase/arylsulfatase and solid phase extraction, drugs were identified by GC/MS. For quantification of flunitrazepam we used its metabolite amino-flunitrazepam; for oxazepam and lorazepam we used the hydrolysed forms of the corresponding drugs. In the hair of 21 subjects tested we found in 20 cases nordazepam, in 15 cases diazepam, in 15 cases oxazepam and in eight cases flunitrazepam with maximal concentrations of 1.8, 2.2, 3.4 and 9.5 ng/mg hair respectively. In addition to these compounds, in subject 11 to 21 we also analyzed for and detected amitriptyline (seven positive), carbamazepine (eight positive), lormetazepam (three positive) and lorazepam (one positive) and found maximal concentrations of 106.0, 13.5, 29.0 and 4.9 ng/mg hair respectively. The comparison of hair analysis versus post-mortem blood and tissues analysis of all the drugs studied shows that in 40 cases, where a positive result was found in blood, the corresponding drug could also be detected in hair in 37 cases. Our results show that hair testing is complementary to classical post-mortem analysis in forensic toxicology.