Abstract
There is growing interest in the detection of bacteria in consumables, for example, in the food and water sectors. In this study, the aim was to produce a polymer-based bacteria biosensor via ROMP (ring opening metathesis polymerization). In the first part of the study, block and random copolymers were synthesized, and their biocidal activities were tested on the glass surface. Interdigitated electrode arrays coated with the polymers possessing the highest activity were used to screen the affinity towards different bacterial strains by monitoring impedance variations in real-time. The polymer-coated electrode could detect gram-positive and gram-negative bacteria strains at a concentration of 107 cfu/mL. The results show that ROMP-based polymer offers bacterial detection and can be used in developing biosensor devices for efficiently detecting pathogenic bacteria.
Highlights
The evolution of antibiotic-resistant microorganisms is one of the biggest global threats [1,2,3,4]
The presence of triphenylphosphonium or hexypyridinium moieties enhances biocidal activities of the copolymer, while thioacetate group favors the binding on the electrode surface
Characteristic signals of oxanorbornene protons were observed at δ 6.49, 5.24, and 2.82 ppm and the protons of the substituent neighbor to the sulphur appeared at δ 3.08 in the 1H NMR spectra (Figure S3)
Summary
The evolution of antibiotic-resistant microorganisms is one of the biggest global threats [1,2,3,4]. According to the Centers for Disease Control and Prevention, Salmonella causes sickness in 1.2 million Americans and leads to 23,000 hospitalization and 450 deaths every year. According to the WHO (World Health Organization), more than 5 million people die each year due to contamination from the food and water supplies by water-borne bacteria. These types of bacteria are known to cause diarrheal diseases22 [7]. There is an increasing need for the development of new and simple methods for the detection of microorganisms in many different fields, such as in food production or water supplies
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