Detection of Azoxystrobin in Environmental Samples using FTIR Spectroscopic Method

Abstract

A new UV-Visible spectrophotometric method for determination of fungicide azoxysrtobin was developed. The method is based on the bromination of azoxystrobin to form dibromoazoxystrobin which react with Potassium iodide, Potassium iodate mixture in the presence of leucomalachite green (LMG) to form a bluish green colored complex. Characterization was done for the synthesis of bluish green colored complex by using UV-Vis spectrophotometer and FTIR methods. As a result, the UV-Visible absorption spectrum was observed at 615 nm. The limits of detection and limits of quantification were observed at 0.0019 µg ml-1 and 0.0059 µg ml-1 respectively. We have also studied the conformational and functional group (such as most characteristic band of O-H stretching frequency observed at 3346.49 cm-1, Bonding N-H symmetrical is 1645.50 cm-1, C=C bending is 691.07 cm-1, Symmetrical stretching C-N is 1493.13 cm-1 and 1404.70 cm-1, C-C stretching and other vibrational is 1059.74 cm-1). Involved in the complexation between azoxystrobin and bromination by FTIR method. This developed method has been successfully applied for the detection of azoxystrobin in various environmental samples. Beer’s law obeyed over the concentration range of 0.5-13 µg mL-1 in final solution volume of 10 ml. The reproducibility assessed by carrying out seven days replicate analysis of a solution containing 5 µg ml-1 of azoxystrobin in a final solution of 10 mL. The molar absorptivity of the colour system is 1.936×106 L mol-1 cm-1 and Sandell’s sensitivity is 0.800 ×10-4 µg cm-2. The relative standard deviation (RSD) for the absorbance value was found to be 1.9%. The suggested method is free from the interference of other toxicant agents. The analytical parameters were optimized and the method was applied to the determination of azoxystrobin in water, soil and food samples.