Detection of avian hematopoietic cell surface antigens with monoclonal antibodies to myeloid cells: Their distribution on normal and leukemic cells of various lineages

Abstract

The isolation and characterization of monoclonal antibodies reacting with cell surface antigenic determinants of normal and leukemic avian hematopoietic cells is described. The antibodies were produced by immunizing mice with normal macrophages, as well as with myeloid cells transformed with the avian acute leukemia viruses MC29, AMV and E26. Eleven antibodies were characterized for their reactivity with a variety of normal and leukemic cells of the myeloid, B- and T-lymphoid and of the erythroid cell lineage. Using several methods, they could be subdivided into five distinct types: 1. I. Four antibodies were specific for the myeloid lineage, predominantly reacting with immature myeloid cells. 2. II. One antibody reacted with mature and immature myeloid cells as well as with T-lymphoid cells. 3. III. Four antibodies reacted with myeloid, erythroid and T-lymphoid cells. 4. IV. One antibody reacted with myeloid as well as with T- and B-lymphoid cells. 5. V. One antibody reacted with all kinds of chicken hematopoietic cells except erythrocytes. The first type of antibodies detected glycoproteins with MWs of 170 and 130 kD. The pattern of antigens precipitated varied with the different monoclonal antibodies of this group. The antibody of the fourth type precipitated a 30 kD polypeptide from extracts of myeloid and lymphoid cells. None of the other antibodies precipitated any detectable proteins.