Detection of Aspergillus Sp Fungus in Tuberculosis Patients with A Treatment Length of 2 Months using the Polymerace Chain Reaction (Pcr) Method

Abstract

This study aims to detect the presence or absence of the Aspergillus sp fungus in Tuberculosis Patients within a 2 month treatment period. This research was carried out in the Microbiology Laboratory at Hasanuddin University Teaching Hospital. Laboratory methods. Samples were obtained from the Tabaringan and Pattingaloang Community Health Centers in Makassar City. Then testing was carried out using culture techniques and polymerace chain reaction techniques. The research results showed that by using the culture method from 30 samples, 9 samples obtained growing media, for 2 samples it was suspected that Aspergillus sp fungus was growing. Then for the polymerace chain reaction (PCR) method, from 30 samples the presence of Aspergillus sp fungus was detected, 4 samples using ASAP primers, then carried out again using ASPU primers (ASPU+Af3r, ASPU+Ni1r, ASPU+Fl2r) which resulted in 1 Aspergillus niger sample. From the results of this research, it can be concluded that the factor that causes the growth of fungus is the long-term administration of anti-tuberculosis drugs which only suppress bacteria from pulmonary tuberculosis.