Abstract
A new method is described for detecting arabis mosaic virus (ArMV) in infected plants. Specific sequences of ArMV-RNA present in total nucleic acid extracts of infected Vitis vinifera or Chenopodium quinoa were initially reverse-transcribed into a complementary DNA (cDNA), then amplified by PCR using specific oligonucleotide-primers. Different primer combinations distinguished between an ArMV infection and an infection with grapevine fanleaf or raspberry ringspot virus. The amount of nucleic acids obtained from 5 mg grapevine leaves resp. 1 mg leaves of Ch. quinoa were sufficient for detecting ArMV.
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