Abstract

A modified Warthin-Starry (mWS) method was developed to stain apoptotic cells in tissue sections. Essentially the classical WS method is applied except for a minor change to prevent fading. In order to clarify the relationship between mWS-positive cells and apoptosis, apoptotic thymic tissues were examined on the rats treated with dexamethasone. The results clearly showed the distribution of mWS-positive cells to coincide well with that for apoptotic cells observed by terminal deoxynucleotidyl transferase (TdT)-mediated d-UTP-biotin nick end labeling (TUNEL) method. An additional characteristic is that nuclei of mitotic thymocytes, with highly condensed chromatin, are always stained. These observations suggest that the mWS method might be useful and convenient to detect condensed chromatin during apoptosis.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.