Abstract
The leucocyte adherence inhibition (LAI) test, previously described for the detection of cell mediated immunity and serum blocking factors associated with murine tumours, has now been adapted for use with human cancer patients. Blood leucocytes from these patients, mixed in vitro with antigenic extracts of tumours of the same type, had their normal adherence to glass surfaces diminished. This inhibition was reversed (blocked) by the addition of the patients' own sera. Both LAI and blocking were tumour-type specific, but showed complete cross-reactivity within each type of tumour (melanoma, colon carcinoma, mammary carcinoma).The LAI test could be of great value in diagnosis and evaluation of treatment, since it seems to reproduce consistently the findings made by more elaborate techniques but has the advantage of being simple, rapid and inexpensive.
Highlights
The leucocyte adherence inhibition (LAI) test could be of great value in diagnosis and evaluation of treatment, since it seems to reproduce consistently the findings made by more elaborate techniques but has the advantage of being simple, rapid and inexpensive
Circulating anti-tumour antibodies have been detected by several methods, notably immunofluorescence (Lewis et al, 1969) and complement-dependent cytotoxicity (Hellstrom et al, 1968); antibody-like blocking factors (BF), which interfere with cell mediated immunity (CMI), are well known in association with tumour growth (Hellstrom et al, 1971b, 1973; Halliday, 1972; Hellstrom and Hellstrom, 1973a, b)
When leucocytes from normal volunteers or patients with cancer were allowed to settle on a glass surface in a haemacytometer, approximately 45-70% of the cells adhered to the surface and withstood a gentle washing procedure
Summary
The LAI test could be of great value in diagnosis and evaluation of treatment, since it seems to reproduce consistently the findings made by more elaborate techniques but has the advantage of being simple, rapid and inexpensive. Specific cell mediated immunity (CMI) has been demonstrated in human patients towards antigens of their own tumours, by lymphocyte cytotoxicity (Hellstr6m et al, 1968, 197 la), lymphocyte transformation (Vanky et al, 1971) and macrophage migration inhibition (Andersen, Bendixen and Schi0dt, 1969). A rapid, simple variation of the macrophage migration test, called leucocyte adherence inhibition (LAI) and characterized in detail with experimental murine tumours (Halliday and Miller, 1972; Halliday, Maluish and Miller, 1973), appears to have many advantages for the immunological investigation of human cancer
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