Abstract
It is critical to differentiate between three distinct staining patterns in the diagnosis of Wegener’s granulomatosis (WG) by indirect immunofluorescence. The first, which is highly specific to WG, is a fine granular diffuse cytoplasmic fluorescent pattern, the second is a nonspecific perinuclear pattern, and the third is a nuclear pattern that must be interpreted as negative. A comparison of cytospin-prepared slides and air-dried drop slides showed that the slides prepared with cytospin substrate were superior in differentiating between the three patterns. Using airdried drop-prepared slides we were unable, in some cases, to distinguish perinuclear from cytoplasmic patterns. Studies using both types of slides indicate that the procedure we developed for the preparation of neutrophil substrate slides offers a better method of identifying antineutrophil cytoplasm antibodies.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
