Abstract
PurposeAntimicrobial resistance (AMR), especially multidrug resistance, is one of the most serious global threats facing public health. The authors proof‐of‐concept study assessing the suitability of shotgun proteomics as an additional approach to whole‐genome sequencing (WGS) for detecting AMR determinants.Experimental DesignPreviously published shotgun proteomics and WGS data on four isolates of Campylobacter jejuni are used to perform AMR detection by searching the Comprehensive Antibiotic Resistance Database, and their detection ability relative to genomics screening and traditional phenotypic testing measured by minimum inhibitory concentration is assessed.ResultsBoth genomic and proteomic approaches identify the wild‐type and variant molecular determinants responsible for resistance to tetracycline and ciprofloxacin, in agreement with phenotypic testing. In contrast, the genomic method identifies the presence of the β‐lactamase gene, bla OXA ‐ 61, in three isolates. However, its corresponding protein product is detected in only a single isolate, consistent with results obtained from phenotypic testing.
Highlights
Antimicrobial resistance (AMR), especially multidrug resistance, is crobial resistance (AMR) mechanisms one of the most serious global threats facing public health
Testing (AST) using phenotypic methdata on four isolates of Campylobacter jejuni are used to perform AMR detection by searching the Comprehensive Antibiotic Resistance Database, and their detection ability relative to genomics screening and traditional phenotypic testing measured by minimum inhibitory concentration is ods, such as the minimum inhibitory concentration (MIC)
Its corresponding protein product is detected in only a culture-based phenotypic AST. Such techniques include genotypic testing with polymerase chain reaction (PCR), transcriptomic testing with quantitative reverse transcription PCR, and single isolate, consistent with results obtained from phenotypic testing
Summary
Both genomic and proteomic approaches identify the wild-type and developed to complement traditional variant molecular determinants responsible for resistance to tetracycline and ciprofloxacin, in agreement with phenotypic testing. Its corresponding protein product is detected in only a culture-based phenotypic AST. Such techniques include genotypic testing with polymerase chain reaction (PCR), transcriptomic testing with quantitative reverse transcription PCR, and single isolate, consistent with results obtained from phenotypic testing. Whole genome sequencing (WGS) provides a comprehensive inventory of an organism’s functional potential, making it an attractive approach for AST. Van Domselaar Department of Medical Microbiology and Infectious Diseases Rady Faculty of Health Sciences, Max Rady College of Medicine University of Manitoba Winnipeg, Manitoba, Canada and rRNA variants, and regulatory mutations in efflux systems.
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