Abstract

The ability of endophytic fungi to produce valuable bioactive compounds when surviving in medicinal herbs. Finding out endophytic fungi originated from Catharanthus roseus with antimicrobial, antioxidant and cytotoxicity activities is important for pharmaceutical development. The isolation was based on the morphology of fungi. Identification was performed by sequencing 18S rRNA that was compared with known genes using Blast search in the combination of phylogenic analysis by using Clustal W and PhyML in GenomeNet. For the antimicrobial test, the agar diffusion method was used. DPPH scavenging assay for the antioxidant activity determination by using spectrophotometry. The cytotoxicity test was carried out by Sulforhodamine B method. LC-MS was applied for predicting components. We isolated the Fusarium oxysporum F01 strain originated from Catharanthus roseus. The aqueous extracts showed inhibition against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 (15.00 ± 0.50 mm), Serratia marcescens ATCC 14756 (9.00 ± 0.87 mm), Vibrio parahaemolyticus ATCC 17802 (15.50 ± 0.87 mm), Escherichia coli ATCC 25922 (12.50 ± 0.50 mm). The antioxidant activity of the extract obtained from the supernatant was determined with an IC50 value of about 11 µg/mL. The extract also showed cytotoxicity effect on liver cancer cell line (HepG2) and breast cancer cell line (MCF-7) with the percentage of inhibition of 84.47 ± 3.18 and 94.69 ± 1.59, respectively. LC-MS was used to point out the presence of pratol, a melanogenesis agent. The study provided more interesting information about Fusarium oxysporum F01 isolated in Catharanthus roseus grown in Vietnam, contributing to pharmaceutical sources in the world.

Highlights

  • Antioxidant associated with their host plant, mimic their activities that can help human in preventing some chemistry and synthesize the same natural diseases were studied much on plant extracts25-28 products and bioactive compounds as these whereas microbial extracts were not investigated originated from their host, and are screened in both the depth and width

  • The cytotoxicity was caused by others compounds in Fusarium oxysporum F01

  • The extract obtained from cultural supernatant showed the weaker cytotoxicity activity than ascorbic acid due to it contained the mixture of antioxidant agents and non-antioxidant agents that interfere the activity

Read more

Summary

Introduction

It is beneficial in form of endophytes – having a mutual relationship to plants, for example, some of these bioactive compounds have been found and extracted vincristine . 18-20 within the plant causing no harm to its host, the antibiotic resistance in microorganisms endophyte is known to generate no apparent symptoms of disease and some of natural plant compounds. Antioxidant associated with their host plant, mimic their activities that can help human in preventing some chemistry and synthesize the same natural diseases were studied much on plant extracts products and bioactive compounds as these whereas microbial extracts were not investigated originated from their host, and are screened in both the depth and width.

Methods
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call