Detection of Antihistamines (Chlopheneramine and Fexofenadine) in blood of fertilized hens eggs embryos by using Thin Layer Chromatography method

Abstract

The aim of the present study was to detect the of antihistamines (Chlorpheneramine and Fexofenadine) in blood of fertilized hens eggs embryos at 12th day of incubation in doses (1mg/ egg injection into air sell) by using thin layer chromatography method (TLC). TLC method cosider simplest form of chromatography techniques and cosider simple,rapid and qualitative technique to identification of drugs in a biological fluids and separation of some organic compoundsfrom living media by estimation arange of retention factor to the drugs and compounds.deteced about Chlorpheneramine and Fexofenadine, the retention factor of Chlorpheneramine (0.289±0.15, 0.272±0.14, 0.285± 0.13) at (30, 60, 120 minute) respectively the color of spot is bright yellow this result is approach to the value of retention factor of standerd solution of Chlorpheneramine (0.295±0.11) and the colore of spot is bright yellow,While the retention factor of fexofenadine (0.214±0.11, 0.222±0.13, 0.219±0.17) at (30,60,120 minute) respectively the colore of spot is bright yellow this resulte is approach to the value of retention factor of standerd solution of Fexofenadine (0.225±0.19) and the color of spot is bright yellow this indicate on fact the antihistamines drugs reached to blood supply during this time and also discussed the nature of this drug of highy lipid solubility and fast absorption by blood vessles of chorioallantoic membrane and reached to blood supply of fertilized hens eggs embryos.The result of current study to detected the antihistamines by using thin layer chromatography (TLC) demonstrated the result of scentific research about TLC metod described here is simple and rapid qualitative technique to identification of chemical compounds .