Abstract
Anthrax is a worldwide zoonosis in animals and human. In Mongolia, the confirmed case of anthrax outbreak is reported every year over the past decade. The prevention and control measure of animal anthrax is vaccination using spore of attenuated Sterne strain, but horse does not get vaccinated in Mongolia. In this study, we constructed the recombinant plasmid for over expression of anthrax protective antigen (PA)/GST fusion protein in pGEX-6P-1 vector and purified the recombinant PA (r-PA) using glutathione Sepharose column under native and denaturing conditions. Since both forms of r-PA were recognized by specific antibody against PA, ELISA system to detect antibody titer in vaccinated bovine serum was constructed. Total of 890 vaccinated cattle serum were collected from 178 cattle at 0, 3, 5, 8 and 12 months’ post vaccination. As negative control, 200 cattle serum from Umnugovi aimag were selected which does not have anthrax foci. All serum was tested by rPA indirect ELISA and, antibody to PA were detected in vaccinated cattle serum but were not detected in negative serum. Therefore, rPA should be used in as monitoring of the anthrax vaccination.
Highlights
Anthrax is a zoonotic disease caused by Bacillus anthracis, gram positive bacteria which forms highly resistant and long-lasting spores in the environment [19]
Human anthrax subsequently occurred after the animals had died and meat was supplied to the people so that some cases of gastric and oropharyngeal anthrax was observed [8,18]
protective antigen (PA) is the most immunogenic of those toxin, PA seems to be an essential component of all vaccine for anthrax including Anthrax vaccine adsorbed (AVA) for human and live spores of Sterne strain vaccine for animals [5,10,19]
Summary
Anthrax is a zoonotic disease caused by Bacillus anthracis, gram positive bacteria which forms highly resistant and long-lasting spores in the environment [19]. It was existed in all continents in Asia including Mongolia, Thailand, India, China [2,11,12,17, 25,26]. Human anthrax subsequently occurred after the animals had died and meat was supplied to the people so that some cases of gastric and oropharyngeal anthrax was observed [8,18]. It is a highly contagious disease, though no incidence of human to human transmission was found and most of infections were cutaneous form.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
