Abstract
The objective of our study was to compare two B. dermatitidis yeast phase lysate antigens [ERC-2, dog Wisconsin; 85, soil Georgia, ATCC 56,920] for detecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. The mean absorbance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. The mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418; Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452; 85 had an absorbance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150; 85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. This study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen.
Highlights
Blastomycosis is a systemic mycosis that can prove fatal, among the immunocompromised
This study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen
ERC-2 lysate antigen exhibited the greatest mean absorbance value (2.359) when the 38 serum specimens from dogs with blastomycosis were assayed using the Enzyme Linked Immunosorbent Assay (ELISA) as compared to a mean absorbance value of 2.189 with the 85 lysate antigen (Figure 1)
Summary
Blastomycosis is a systemic mycosis that can prove fatal, among the immunocompromised. Blastomycosis is caused by the inhalation of the thermally dimorphic fungal agents Blastomyces dermatitidis. Though it has never been directly observed growing in nature, it is thought to grow there as a cottony white mold, similar to the growth seen in artificial culture at 25 ̊C (77 ̊F)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
