Abstract
Enzyme linked immunosorbent assay (ELISA) was applied to detect the antibody of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus (LCDV). Purified Japanese flounder LCDV was used as a capture antigen of ELISA. Monitoring the immune response of Japanese flounder to LCDV was done using the sera obtained from apparently healthy, LCD-diseased and LCD-recovered fish. ELISA absorbances of these three groups were clearly different. Using the serum with high ELISA absorbance, the optimum ELISA condition was set up. Apparently healthy fish were injected with inactivated LCDV. One to three months after the injection, the antibody against LCDV was detected by ELISA established for LCDV and their ELISA antibody titer increased during that period.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
