Detection of antibodies to herpes simplex virus type 1 and human cytomegalovirus in 178 neonates

Abstract

Objective To understand serum positive rates of herpes simplex virus type 1 (HSV-1) and human cytomegalovirus (HCMV) in 178 neonats and their relationship with clinical manifestation of premature infants.Methods A total of 178 neonatal serum samples were collected,including 114 umbilical cord sera from normally delivered newborns and 64 blood sera from premature infants.IgG and IgM antibodies to HSV-1 and HCMV were detected with ELISA.The x2 test was adopted for the comparisons.Results In 178 neonats,HSV-1 IgG and IgM positive rates were 83.70％ and 0.00％,HCMV IgG and IgM positive were 94.38％ and 0.56％,respectively.In 114 normally delivered newborns,HSV-1 IgG and IgM positive rates were 78.07％ and 0.00％,HCMV IgG and IgM positive were 94.74％ and 0.88％,respectively.The positive rate of both HSV-1 IgG and HCMV IgG was 73.68％.In 64 premature infants,HSV-1 IgG and IgM positive rates were 93.75％ and 0.00％,HCMV IgG and IgM positive rates were 93.75％ and 0.00％,respectively.The positive rate of both HSV-1 IgG and HCMV IgG was 87.50％.The HCMV lgG positive rates between premature and normally delivered neonates were not statistically significant (x2 =0.07,P ＞ 0.05 ) ;while HSV-1 IgG positive rate and both HSV-1 IgG and HCMV IgG positive rate in premature neonates were significantly higher than those in normally delivered newborns (x2 =7.38,P ＜ 0.05 ; x2 =4.65,P ＜ 0.05 ).The premature infants with HSV-1 IgG and HCMV IgG positive had higher risk of premature rupture of membranes,placental abruption,oligohydramnios,amniotic fluid contamination and skin abnormalities.Conclusions The positive rates of HSV-1 IgG and HCMV IgG are high in neonates.The premature infants with HSV-1 IgG and HCMV IgG positive may be associated with premature rupture of membranes and other clinical manifestations. Key words: Cytomegalovirus ; Herpesvirus 1,human ; Enzyme-linked immunosorbent assay ; Infant,newborn ;