Detection of antibiofilm formation by sliver nanoparticles created by tetracycline antibiotic

Abstract

Silver conjugated Tetracycline, was created and characterized by ultraviolet visible spectrophotometry (UV-vis), Fourier transform infrared (FT-IR), X-Ray Diffraction Patterns (XRD). Using antibacterial assays, the effects of tetracycline alone and drugs-conjugated with silver nanoparticles were tested against Gram-ve Pseudomonas aeruginosa isolates by well diffusion assay. The UV-vis spectra of silver-drug Nano conjugates showed a characteristic surface Plasmon resonance band in the range of 400–450 nm. FTIR analysis demonstrated the involvement of Hydroxyl groups in both drugs in the stabilization of silver nanoparticles. (XRD) showed that they cubic structure of silver and, antibacterial assays showed that biosynthesis silver nanoparticle conjugation enhanced antibacterial potential of Tetracycline compared with drug alone.