Detection of ANO1 mRNA in PBMCs is a promising method for GISTs diagnosis

Haini Li,Ancheng Wu,Wuhui Zhu,Zongtao Liu,Yufen Yan,Feng Hou,Congxiao Zhang,Jinpeng Cao,Shaoyun Cheng

doi:10.1038/s41598-019-45941-2

Abstract

ANO1 is a calcium-activated chloride channel protein that has been used to diagnose GISTs after tissue biopsy. Recently, ANO1 mRNA amplification in the blood has received considerable attention as a useful method for the diagnosis of GISTs. The aim of this study was to evaluate the diagnostic ability of ANO1 mRNA in distinguishing GIST patients from healthy subjects. We constructed a logistic regression model for examining the diagnostic ability of ANO1 mRNA in comparison with conventional tumor markers, including CEA, CA199, and CA724. Our results showed that ANO1 mRNA was significantly amplified in PBMCs, the average expression level and range of ANO1 mRNA in the blood were increased along with the expression of ANO1 in the tissues, and the extent of amplification of ANO1 was associated with tumor size. In addition, ROC curve analysis showed that ANO1 mRNA in the blood had the highest specificity when compared with conventional tumor markers. Moreover, a combined analysis with ANO1 mRNA and conventional tumor markers had the highest sensitivity in diagnosing GISTs. Our study indicated that detection of ANO1 mRNA in PBMCs is a promising method for diagnosis of GISTs in vitro.

Highlights

The anoctamin-1 (ANO1) gene is localized to chromosome 11q13, a locus that is frequently amplified in a series of human cancers, such as bladder cancer, breast cancer, and head and neck squamous cell carcinoma (SCCHN)[10]
We found that amplification of the ANO1 mRNA in peripheral blood mononuclear cells (PBMCs) is correlated with tumor size of GISTs, and the average expression level and range of ANO1 mRNA in the blood are increased along with the expression of ANO1 in the tissues
To confirm the observation of upregulation of ANO1 mRNA in PBMCs from patients with GISTs, we examined the expression of ANO1 mRNA in the blood of patients with GISTs27

Summary

Introduction

The anoctamin-1 (ANO1) gene is localized to chromosome 11q13, a locus that is frequently amplified in a series of human cancers, such as bladder cancer, breast cancer, and head and neck squamous cell carcinoma (SCCHN)[10]. This study aimed to explore the diagnostic role of ANO1 in detection of GISTs. this study aimed to evaluate the combined diagnostic efficacy of conventional biomarkers, including CEA, CA199, and CA724, and ANO1 for the diagnosis of GISTs. In this study, we found that amplification of the ANO1 mRNA in PBMCs is correlated with tumor size of GISTs, and the average expression level and range of ANO1 mRNA in the blood are increased along with the expression of ANO1 in the tissues. A combined analysis with ANO1 mRNA and conventional tumor markers can significantly increase the diagnostic ability

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Conclusion