Abstract
It is known that five nitroaniline and dinitroaniline isomers including 2‐nitroaniline, 3‐nitroaniline, 4‐nitroaniline, 2,4‐dinitroaniline and 2, 6‐dinitroaniline were detected in the acidic wastewater and in printing and dyeing wastewater. In this study, Yeast R112 was inoculated in the biosolid(biosludge) to determine the growth pattern and tolerance level to aniline isomer derived toxicants. Biosolid was obtained from the City of Eagle Pass Waste Water processing plant. The biosolid represent organic and inorganic remains of raw sewage subjected to oxygen treatment in the aeration chamber. Yeast R112 showed substantial growth in the undiluted biosludge, but not in the higher dilutions. Virtually no Yeast R112 was detected in the 10–2, 10–3, 10–4, and 10–5 dilutions. Also, antibiotic sensitivity was performed by Kirby‐Bauer disk diffusion antibiotic sensitivity testing. Yeast R112 strain resistant to Aniline isomers showed selective growth inhibition by streptomycin 50μg/disc. Whereas a normal growth pattern was observed in the presence of Methicillin, Chloramphenicol, and Kanamycin. Taken together, Yeast R112 strain showed resistance to Aniline isomers present in the sewage water. Also, Yeast R112 strain showed sensitive to Streptomycin.
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