Detection of amplified SRY gene by a novel electrochemical biosensor based on gold nanoparticles

Abstract

This paper describes an electrochemical hybridization biosensor that investigates DNA hybridization on the modified Glassy Carbon Electrode (GCE). GCE was modified by a mixture of Au nanoparticles (AuNPs) and calf thymus DNA (ctDNA). SH-ssDNA was self-assembled on a modified electrode, which is a segment of the sex determining region of the Y (SRY) gene. This electrode can detect the complementary and non-complementary strand of SRY gene, by Methylene Blue (MB), as an electroactive label, and the Differential Pulse Voltammetry (DPV) technique. The modified GCE for the complementary DNA had a linear dynamic range of 0.5–30.0 ppm. This is the first report of sex determination by the electrochemical biosensor of a Polymerase Chain Reaction (PCR) product. Also, this biosensor showed stability on thermal denaturation for five sequential denaturation processes.