Abstract
Legionella pneumophila (L. pneumophila) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying and monitoring of Legionella in the environment using conventional and modern technologies. The present study aims to compare detection of L. pneumophila in water samples using both culture and PCR techniques. A pre-enriched contaminated water sample was split into 13 subsamples. Culture and PCR tests were done from the subsamples after different intervals. The results showed a positive PCR result for L. pneumophila after 8 h of incubation. Also, L. pneumophila was detected by culture on non-selective BCYNE agar and selective GPVC agar after 5 and 6 days of incubation respectively. There was no significant difference between the non-selective BCYE- and the selective GVPC method. The PCR procedure was found more sensitive and differed significantly from the conventional selective GVPC method in isolation of L. pneumophila from water samples. It was concluded that pre-enrichment incubation allows the detection of L. pneumophila by PCR within a maximum of 12 h from the collection of water samples.
Highlights
Legionellae is a Gram-negative, aerobic, non-spore-forming, encapsulated bacillus
L. pneumophila was detected by culture on non-selective BCYNE agar and selective glycine-polymyxin B-vancomycin-cycloheximide (GPVC) agar after 5 and 6 days of incubation respectively
There was no significant difference between the non-selective buffered-charcoal-yeast extract (BCYE)- and the selective GVPC method
Summary
The genus Legionella comprises approximately 53 species They are ubiquitous in natural fresh water environments such as lakes and streams. Bacterial growth in the aqueous environment is sometimes exposed to stresses due to unfavorable conditions, so they adapt to such conditions by entering a temporarily non-cultivable state. The application of PCR for the detection of L. peumophila DNA is useful for the early monitoring and controlling the organisms in water samples. This technique is cost-effective and reliable and has been described as useful tools for the detection of L. pneumophila in clinical and environmental samples. The present study aims to compare detection of L. pneumophila in pre-enrichment water samples using both culture and PCR techniques
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
