Abstract
American foulbrood, a virulent disease of honeybees, is caused by Bacillus larvae. A murine hybridoma which secretes an IgM monoclonal antibody reactive with B. larvae was derived and an indirect enzyme-linked immunosorbent assay (ELISA) using this monoclonal antibody was evaluated for use in detection of the pathogen. The ELISA detected B. larvae strains from Alberta. and British Columbia, Canada, and from the United States but did not detect six other Bacillus species. The ELISA also detected the presence of B. larvae in field samples collected from commercial apiaries. Results of testing against cultures of B. larvae and infected bee larvae from hives indicate that the monoclonal antibody has, in conjunction with the indirect ELISA technique, potential for use in the diagnosis and confirmation of American foulbrood in commercial apiaries and in honeybee pathology research. Key words: American foulbrood, Bacillus larvae, honeybee disease.
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