Abstract
Aflatoxins are secondary metabolites of Aspergillus flavus and Aspergillus parasiticus. These fungal species are the most dangerous and common toxin group causing food contamination. Aflatoxin has high toxicity and can cause cancer to humans and animals. The quantitative detection of aflatoxin in food, therefore, plays a very important role. However, in practice, due to low concentrations, aflatoxin detection analysis methods need to be highly sensitive and simple to apply. In this report, the fluorescence resonance energy transfer method (FRET) adopts the donor–acceptor interaction of aflatoxin B1. The CdSe/ZnS quantum dot detection of aflatoxin B1 will be presented wherein the aflatoxin B1 concentration can be determined from the changes in fluorescence lifetime or fluorescence intensity. A fluorescence lifetime calibration curve versus aflatoxin B1 concentrations was established. Test results of aflatoxin B1 determination in maize in Vietnam by FRET method are consistent with the results of aflatoxin B1 determination by HPLC based on ppm concentration.
Highlights
Aflatoxins are cancerous secondary metabolites from Aspergillus flavus and Aspergillus parasiticus.Due to their high toxicity and carcinogenic potential, they are a high concern for the safety of food worldwide
CdSe/ZnS quantum dots (QDs) were synthesized by the successive ionic layer absorption and reaction (SILAR) method
There are four criteria that must be satisfied in order for fluorescence resonance energy transfer (FRET) to occur
Summary
Aflatoxins are cancerous secondary metabolites from Aspergillus flavus and Aspergillus parasiticus. Due to their high toxicity and carcinogenic potential, they are a high concern for the safety of food worldwide. They can be found in various agricultural products such as grains, nuts, spices, etc. A third subset, M1 and M2, arise as metabolic products when dairy cattle eat B contaminated grains [1,2,3,4]. Aflatoxin B1 (AFB1) is the most common and most widespread [5,6] in the world and accounts for 75% of all aflatoxins contamination of food and feeds [7]
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