Abstract
This study aimed to determine Aeromonas spp . in raw milk and some milk products. A total of 100 raw milk, kareish cheese, ice cream and baladi yoghurt (25 samples, each) were collected from different dairy shops and street peddlers in Assiut city, Egypt and were bacteriologically examined for presence and count of Aeromonas spp . The incidences of counted Aeromonas spp . in raw milk, kareish cheese, ice cream and baladi yoghurt were 36, 32, 24 and 0.0%, respectively, with average counts of 1.0×105, 3.2×104, 5.0×102 and < 100 cfu/ml, respectively. The incidences of counted Aeromonas hydrophila, in raw milk, kareish cheese and ice cream were 16, 12 and 8%, while for Aeromonas caviae, the incidences were 12, 16 and 12%, respectively. Moreover, the incidences of counted Aeromonas sobria in raw milk, kareish cheese and ice cream were 8, 4 and 4%, respectively. Baladi yoghurt samples were negative for Aeromonas spp. in this study. All the recovered Aeromonas hydrophila organisms were confirmed by PCR assay for the presence of 16S rRNA gene and 100% of the tested strains harboured this gene. The aerA and ahh1 virulence genes were present in Aeromonas hydrophila in percentages of 66.67 and 77.78%, respectively. All the recovered Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria strains, in this study, exhibited 100% virulence properties on bases of proteolytic, lioplytic, psychrotrophic and β-haemolytic activities. The recovered Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria exhibited 100% resistance towards Ampicillin, Amoxicillin and Erythromycin antibiotics, while, they exhibited 100% sensitivity towards Ciprofloxacin. The public health hazards of occurrence of Aeromonas spp. in milk and its products as well as the suggestive control measures were discussed.
Highlights
Isolation of Aeromonas hydrophila from water and food sources, and the increasing resistance of thisMilk is an excellent medium for the growth of numerous microbes which produce consequential spoilage of the milk and various milk products or food-borne pathogens to the consumers (Oliver et al, 2005)
Due to the public health hazard of Aeromonas species, this study aimed to detect these microorganisms in raw milk and some milk products consumed in Assiut city, Egypt
Presence of Aeromonas in a high level in raw milk samples in this study is indicative to bad hygienic measures of milk production and distribution (Korashy, 2006)
Summary
Isolation of Aeromonas hydrophila from water and food sources, and the increasing resistance of this. Milk is an excellent medium for the growth of numerous microbes which produce consequential spoilage of the milk and various milk products or food-borne pathogens to the consumers (Oliver et al, 2005). Scoaris et al (2008) showed that, Aeromonads are efficient colonizers of surfaces and are an important constituent of bacterial biofilms in both water distribution systems and food processing environments. Aeromonas hydrophila and Aeromonas caviae are considered major pathogens most commonly implicated in human intestinal infections (Van Gravenitz, 2007) and account for more than 95% of all blood-borne infections (Ghenghesh et al, 2008). Five types of diarrhea of Aeromonas related gastroenteritis, secretory (acute watery diarrhea often with vomiting), dysenteric (accompanied by blood and mucus in the stool), chronic (lasting longer than 10 days), choleric
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