Detection of adhesion genes, biofilm formation of uropathogenic Escherichia coli isolated from women suffering from UTI

Abstract

Urinary tract infections (UTIs) are the most common bacterial infections, and uropathogenic Escherichia coli (UPEC) is the most common bacteria that causes them. The study exposed the distribution of virulence factors from the likes of adhesin genes, and the relation with biofilm production in E. coli isolated from women with UTIs. We evaluated some virulence factor genes (VFGs) of 30 isolates of uropathogenic E. coli.. The tube method and microtiter plate were used to detect the capacity of the UPEC to form biofilm. The biofilm production ability of the UPEC isolates showed that 19 isolates were biofilm producer in TM method (63.3%) and the remaining 11(36.6) isolates not able to form biofilm while MTP showed that 25 (83%) isolates were producers, 6 being strong producers, 19 moderate while the other 5 were weak. Multiplex PCR was implemented for the detection of fimH (465bp) and pap (28bp). The results showed that all 15 i isolates had fimH (100%) and pap was present in 12(80%) of the total isolates. As for sfa and ompT genes, 244bp and 559bp product size respectively, the results showed that 7(46.6%) isolates had sfa while 10(66.6%) isolates detected ompT gene. The current study showed that the genes most prevalence were fim, pap and ompT, the association between biofilm production and VFG, UPEC isolates were classified into two groups: first group are strong (+ +) biofilm producers and second are moderate (+) producers. According to present results the prevalence of sfa in group I was 83% and 22%in group II respectively, while ompT in group I was 33% and 88% in group II. Furthermore, pap was prevalent in 100% of group I and 77% in group II, while fimH was present in 100% of both groups.