DETECTION OF ADENOVIRUS ANTIGEN BY A SURFACE-ENHANCED RAMAN SCATTERING ENZYME-LINKED IMMUNOSORBENT ASSAY

A M Kudryashova,E B Faizuloev,A G Galstian,G V Lisichkin,A Yu Olenin,V V Zverev,O V Borisova

doi:10.36233/0372-9311-2018-3-25-31

Abstract

Aim. Study of the possibility of adenovirus antigen detection by recording of surface-enhanced Raman scattering (SERS) spectra of enzyme oxidized product of 3,3',5,5'-tetramethylbenzidine. Materials and methods. Clinical fecal samples containing adenoviruses, group A rotaviruses, noroviruses and healthy children samples, as well as laboratory strains of adenoviruses with a titer of 5 — 6 lg TCD50/ml were used. Sandwich immunoassay was used, the Raman spectra were recorded by a Raman spectrometer (532 nm) after incubation with silver nanoparticles. Results. The concordance of the adenovirus detection results was obtained in comparison with the enzyme immunoassay method with colorimetric detection and PCR. Conclusion. The possibility of TMB+ using as a SERS reporter and silver nanoparticles as a SERS substrate for the detection of adenovirus antigen in complex biological samples was shown.

Highlights

Liang J., Liu H., Huang C. et al Aggregated silver nanoparticles based surface-enhanced raman scattering enzyme-linked immunosorbent assay for ultrasensitive detection of protein biomarkers and small molecules
Поступила 20.12.17 Контактная информация: Борисова Ольга Васильевна, к.х.н., 105064, Москва, М

Summary

Introduction

Vengatesan D., Raj G.D., Raja A. et al Detection of rabies virus antigen or antibody using flow cytometry. ВЫЯВЛЕНИЕ АДЕНОВИРУСНОГО АНТИГЕНА МЕТОДОМ ТВЕРДОФАЗНОГО ИММУНОФЕРМЕНТНОГО АНАЛИЗА С ГКР-ДЕТЕКЦИЕЙ СИГНАЛА Изучение возможности выявления антигена аденовируса с детекцией спектров гигантского комбинационного рассеяния (ГКР) окисленной формы 3,3',5,5'- тетраметилбензидина (ТМБ+) как продукта иммуноферментной реакции. Иммуноферментный анализ проводился в сандвич-формате, при проведении ГКР-детекции после стадии окисления ТМБ в реакционную смесь вносили наночастицы серебра и проводили измерение спектров на рамановском анализаторе при длине волны лазера 532 нм.

Results

Conclusion