Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism.

Pavel A Erokhov,Anna A Kolacheva,Natalia P Sharova,Yulia O Nikishina,Alexandra S Radchenko,Yulia V Lyupina

doi:10.18632/oncotarget.20208

Pavel A Erokhov, Anna A Kolacheva + Show 4 more

Open Access

https://doi.org/10.18632/oncotarget.20208

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Journal: Oncotarget	Publication Date: Aug 10, 2017
Citations: 12	License type: cc-by

Affiliation: Koltzov Institute of Developmental Biology

Abstract

The aim of this work was to detect changes in proteasome pools of brain parts of August rats with monoamine metabolism violations in comparison with that of control Wistar rats. To reveal active proteasome structures, a method of native electrophoresis for the analysis of crude tissue fractions was developed. By means of this method and following Western blotting, the most pronounced changes in reorganization of proteasome structures were detected in proteasome pool of the brain cortex of August rats. Main findings are the enhanced expression of immune proteasome subtypes containing proteolytic subunit LMP2 and activator PA28αβ as well as immune proteasome subtypes containing proteolytic subunit LMP7 and activator PA700 and simultaneously decreased expression of subtypes with subunit LMP2 and activator PA700 in the brain cortex of August rats compared to that of Wistar rats. These results were indirectly confirmed by SDS PAGE method followed by Western blotting, which showed the increased quantities of immune subunits and proteasome activators in the brain cortex of August rats compared to that of Wistar rats. Immune proteasomes were revealed by immunohistochemistry in neurons, but not in glial cells of August and Wistar rat cortex. The detected reorganization of proteasome pools is likely to be important for production of special peptides to provide the steady interaction between neurons and adaptation of central nervous system to conditions caused by monoamine metabolism deviations.

Highlights

The study of adaptive mechanisms developing in the brain under stress conditions is tightly related to the elucidation of neural plasticity phenomenon
Main findings are the enhanced expression of immune proteasome subtypes containing proteolytic subunit LMP2 and activator PA28αβ as well as immune proteasome subtypes containing proteolytic subunit LMP7 and activator PA700 and simultaneously decreased expression of subtypes with subunit LMP2 and activator PA700 in the brain cortex of August rats compared to that of Wistar rats
The detected reorganization of proteasome pools is likely to be important for production of special peptides to provide the steady interaction between neurons and adaptation of central nervous system to conditions caused by monoamine metabolism deviations

Summary

Introduction

The study of adaptive mechanisms developing in the brain under stress conditions is tightly related to the elucidation of neural plasticity phenomenon. In the motor cortex of August rats, the level of biogenic amines and their metabolites is known to be higher than in the midbrain reticular formation in contrast to Wistar rats [5, 6]. Despite of this imbalance, www.impactjournals.com/oncotarget the exchange between the cortex and subcortical structures that manages function of the cerebral cortex, in August rats remains intact [7]. August rats show higher inherent basal activity of stress-limiting systems (NO synthase and heat shock proteins, HSPs) accompanied by the increased resistance to the acute emotional stress in comparison with Wistar rats [8,9,10]

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