Detection of abnormal findings in human RBC in diagnosing G-6-P-D deficiency Haemolytic Anaemia using image processing

Abstract

Red Blood Corpuscles are one of the most vital cellular components of human circulatory system. In normal physiological condition, an RBC is circular in front view and biconcave in side view. Also it is 7.5 μm in diameter and 2 μm in thickness in terms of size. The normal morphology of an RBC undergoes some specific changes due to the consequence of different pathological abnormalities. One of such disease is Haemolytic Anaemia due to Enzymopathy (like G-6-P-D deficiency) where the RBCs take uneven edged `Bitten Apple' like shape. Here in this paper, correct identification of change in normal parameters of red blood cells in an anaemic blood sample has been presented using different image processing tools and techniques. Here the blood smear image is preprocessed by using Weiner filter and Sobel Edge detection method is applied to find the boundary of the corpuscles. Also the region selection is done by which a metric is formulated to determine abnormal shape of the corpuscles. The purpose of this paper is to highlight this medico-technical aspect only.