Detection of a Traumatic Brain Injury Biomarker at the 10fg/mL Level.

Abstract

The detection of minute amounts of protein biomarkers in body fluids is believed to provide early diagnosis and prognosis of mild traumatic brain injury (mTBI). An ultrasensitive detection method was used to detect S100B, the most studied potential marker for the diagnosis of mTBI. The detection method was a modified electrochemical immunoassay technique that provides voltage controlled intrinsic current signal amplification. The sandwich immune complex of S100B was formed on the working electrode of the screen-printed electrode. The gating voltage provides amplification of the current signal that flows through the complex. S100B was spiked in human serum. The limit of detection of S100B in human serum was 10fg/mL. The calibration curves cover four orders of magnitudes from 10fg/mL to 10ng/mL. The specificity of the detection was demonstrated using TAU protein, which is another marker for mTBI. The results reported in this work using the field effect enzymatic detection (FEED)-based immunoassay indicate the feasibility of using this method for the detection of extremely low concentrations of markers of mTBI in human serum. This method can be developed as a platform for a range of markers of mTBI.