Abstract
A region of the coat protein (CP) gene of grapevine fanleaf virus (GFLV) was detected by RT-PCR in the nematode vector Xiphinema index. The reverse transcription step was performed with total RNA extracted from viruliferous X. index. From the synthesized first cDNA strand, an 810-nucleotide fragment (nt 2515-3324 of GFLV-RNA2) was amplified by two 25-mer primers designed in the CP gene. Specific detection was successfully accomplished with samples of one or 10 viruliferous nematodes. Preliminary analysis and comparison of the 207-nt sequence of a stretch (nt 2549-2755 of GFLV-RNA2) of five different RT-PCR amplified fragments, obtained from 10 nematodes of a same sample in a diseased vineyard, indicated substantial variations in the GFLV CP gene
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
