Detection of a High Frequency of e1a3/BCR-ABL in Chronic Myeloid Leukemia Patients of Sulaimania Province by RT-PCR

Abstract

Introduction: In order to detect the mutation variants of BCR-ABL fusion genes that lead to leukemia in human, 30 blood samples from chronic myeloid leukemia patients and 10 blood samples from apparently healthy people (as control) were collected and tested hematologically, morphologically and by amplification of the fusion mRNA by RT-PCR. The e1a3 variant was detected by amplification of 309 bp from the fusion of exons 1 of bcr and exon 3 of abl gene. Materials and method: Application of RT-PCR assay to detect the prevalence and frequency of ela3 and ela2 variants in CML patients and in routine diagnosis of the most common leukemia translocations. Results: The RT-PCR showed that out of 30 patients, 23 (76.6%) were with e1a3 mutation variant and 7 (23.3%) were negative for e1a2 and e1a3 variants. All the 10 control samples showed no mutations related to these variants. Conclusion: The e1a3 variant was detected by amplification of 309 bp from the fusion of exons 1 of bcr and exon 3 of abl gene and the RT-PCR showed that out of 30 patients, 23 (76.6%) were with e1a3 mutation variant and 7 (23.3%) were negative for e1a2 and e1a3 variants, while the 10 control samples showed no mutations related to these variants.