Detection of 1-Hydroxypyrene by Extractive Electrospray Ionization Mass Spectrometry

Abstract

A method for the detection of 1-hydroxypyrene(1-OHP) using extractive electrospray ionization mass spectrometry(EESI-MS) has been investigated,including the ionization of 1-OHP in a home-made EESI source,effects of the composition of ESI solvent and sample solvent on signal intensity,and semi-quantification analysis.The results indicated that,1-OHP can be effectively extracted and ionized in the EESI source,producing the quasi-molecular ion -(m/z 217) and MS/MS fragment ion [M-H-CO]-(m/z 189);when water,methanol,ethanol,n-propanol and n-butanol were used as ESI solvents,respectively,the highest signal to noise ratio(S/N) was achieved while using methanol as ESI solvent;with the increase of methanol content in sample solution,the signal intensity increased significantly,and the signal intensity of m/z 217 in methanol was more than 200 times higher than that in water.The signal intensity of m/z 217was linearly related with the concentration of 1-OHP in methanol within the concentration range of 10-200 μg/L;the correlation coefficient(R) was 0.982,the relative standard deviations(RSD) were 3.4%-14.0%(n=5),the quantitative detection limit(LOD) for 1-OHP in methanol was about 10 μg/L(S/N=10),and the analytical time of each detection was less than 0.5 min.