Abstract
Sensor devices that can be fabricated on a flexible plastic film produced at a low cost using inkjet-printing technology are suitable for point-of-care applications. An organic field-effect transistor (OFET)-based biosensor can function as a potentiometric electrochemical sensor. To investigate the usefulness of an OFET-based biosensor, we demonstrated the detection of 1,5-anhydroglucitol (1,5-AG) and glucose, which are monosaccharides used as biomarkers of diabetes. An OFET-based biosensor combined with a Prussian blue (PB) electrode, modified with glucose oxidase (GOx) or pyranose oxidase (POx), was utilized for the detection of the monosaccharides. When the GOx- or POx-PB electrode was immersed in glucose solution at the determined concentration, shifts in the low-voltage direction of transfer characteristic curves of the OFET were observed to be dependent on the glucose concentrations in the range of 0–10 mM. For 1,5-AG, the curve shifts were observed only with the POx-PB electrode. Detection of glucose and 1,5-AG was achieved in a substrate-specific manner of the enzymes on the printed OFET-biosensor. Although further improvements are required in the detection concentration range, the plastic-filmOFET-biosensors will enable the measurement of not only diabetes biomarkers but also various other biomarkers.
Highlights
A biosensor, which allows for the detection of a target molecule through biochemical reactions mediated by biofunctional materials like enzymes and antibodies, is in demand for environment monitoring, food analysis and healthcare [1,2,3]
We demonstrate the detection of 1,5-anhydroglucitol (1,5-AG) and glucose, which are monosaccharides used as diabetes biomarkers [20,21,22], using two organic field-effect transistor (OFET)-based biosensors combined with a Prussian blue (PB) electrode modified with glucose oxidase (GOx) or pyranose oxidase (POx)
The OFET-based biosensor with DTBDT-C6 as an organic semiconductor offers the advantage of higher sensitivity compared to our previous OFET sensor, which was equipped with another organic semiconductor poly(2,5-bis(3-hexadecylthiophen-2-yl)thieno[3,2b]thiophene)(PBTTT-C16 ) with ON–OFF currents of a transistor in the order of 10−9 to 10−6 A [15,17]
Summary
A biosensor, which allows for the detection of a target molecule through biochemical reactions mediated by biofunctional materials like enzymes and antibodies, is in demand for environment monitoring, food analysis and healthcare [1,2,3]. Electrochemical measurements, including potentiometry and amperometry, allow for the construction of inexpensive, small and multi-channel biosensors by combining an enzyme electrode and an amplification circuit [13,14]. We have previously reported the OFET-based detection of biomolecules with a sensor for saccharides [17], lactic acid [18] and proteins [19] by using a chemical receptor of phenylboronic acid, an enzyme of lactate oxidase and an antibody
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