Abstract
This chapter describes some of the available methods to assess EPS production in bifidobacteria, being largely based on those developed for the same purpose for members of the lactic acid bacteria group. The first step is detection of putative EPS-producing bifidobacteria based on a mucoid and/or ropy phenotype. Next, a basic procedure is described for the isolation of the glycan polymer based on the release from bifidobacterial cells grown and collected from the surface of agar-MRSc ("crude EPS"), followed by a purification procedure intended to remove other bacterial macromolecules (DNA and proteinaceous material) to generate "purified EPS." Finally, several methods used for quantification and physical-chemical characterization of isolated/purified polysaccharide are outlined.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
