DETECTION FIMH AND PAPG GENES OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIA ISOLATED FROM GALLSTONE , URINE STONE AND GASTROINTESTINAL CANAL BY POLYMERASE CHAIN REACTION (PCR) .

Abstract

During this work twenty six isolates of Escherichia coli were collected (12 from gallstones , 10 from urin stones and 4 from gastrointestinal canal) and 14 isolates of Klebsiella pneumonia (4 from gallstones , 6 from urine stones and 4 from gastrointestinal canal) from Al-Ramadi Teaching Hospital. The resulte about Detection of fimH gene which are responsible for coding to Mannose sensitive fimbriae and three types of papG genes which are responsible for coding to Mannose resistance fimbriae showed (50-60)% of E. coli and (83.33%) of K. pneumoniae both of bacteria were isolated from urine stones only have fimH gene. E. coli isolates from gallston have papGI gene (16.67%) , papGII (50%) and papGIII gene (100%), While E. coli isolates from urine stone have papGII (20%) and papGIII (70%), While E. coli isolates from gastrointestinal canal have papGII (50%) and papGIII (25%), but K. pneumonia isolates didn,t possess any of three papG genes.