Abstract
Bio-insecticides based on the spore forming bacterium, Bacillus thuringiensis (Bt) have been used for commercial scale for the past 40 years. Bt is a Gram-positive soil bacterium that forms insecticidal crystal proteins (ICPs) during sporulation; it has been characterized as an insect pathogen. Vegetative insecticidal protein (VIP) is a newly discovered family of toxin protein isolated from Bt. A hundred strains of localBacillus thuringiensis were isolated from soil and dead larvae, identified by 16S rRNA and screened for the presence of vip1 and vip2 genes by polymerase chain reaction (PCR) amplification, with only four strains producing the desired bands of Vip1 and Vip2. The amplified fragments were cloned in pGEM-vector, sequenced and analyzed. The nucleotide sequences of vip1 (2.3 kb) and vip2 (1.3 kb) were given Gene-bank accession numbers: JN008908 and JN035904, respectively. Vip1 and Vip2 showed 99% homology with the previously isolated genes. Key words: Bacillus thuringiensis, vegetative insecticidal proteins (vip1 and vip2), 16S rRNA, cloning.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
