Abstract

The aim of this study was to isolate motile aeromonad strain of common carp (Cyprinus carpio) which was obtained from farms around Baghdad and identification of the isolate by using Analytical profile index API20E. Experimental infection was carried out by intramusculer injecting LD50 does 0.3× 108.66 cell/fish into common carp Cyprinus carpio L. Infected fish after 14 days of injection showed hemorrhage and ulceration on the body surface, eye abnormalities and accumulation of red-colored ascetic fluid . Macroscopically pale liver with hemorrhagic and necrotic spots was seen and kidney showed liquefaction. Microscopically severe necrotic changes in muscular structure and internal organs with intense polymorphonuclear cell. Infiltration characterized by of pyknosis of nuclei with presence of cellular detritus were seen.

Highlights

  • Aeromonas hydrophila has been recovered from a wide range of freshwater aquarium and cultured fish species worldwide

  • A. hydrophila has been described as the dominant infectious agent of ‘fish-bacterial-septicemia’ in freshwater cultured cyprinid fishes, mainly common carp

  • This paper describes the lethal dose 50% (LD50) of pathogenecity of A. hydrophila on common carp

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Summary

Introduction

Aeromonas hydrophila has been recovered from a wide range of freshwater aquarium and cultured fish species worldwide A. hydrophila has been described as the dominant infectious agent of ‘fish-bacterial-septicemia’ in freshwater cultured cyprinid fishes, mainly common carp. Aeromonas hydrophila was known to produce toxic substances evoking hemorrhage and necrosis (11). Those in vivo and in vitro studies confirmed the bacterial toxin including enzymes such as protease and elastase. This bacterium produces βtype hemolysin on blood agar plates (12). An infection of A. hydrophila producing strong β-hemolysin was found among common carps, Aerolysin and hemolysin genes are reported to be the putative virulence genes of A.hydrophila (13)

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