Detection and Quantification of Viral and Satellite RNAs in Plant Hosts

Abstract

Northern blotting is a valuable method for detection and quantification of RNA in the field of virology. Although many methods including a various versions of polymerase chain reaction have been developed over the years, Northern blotting has been still considered as a useful and effective method for the analysis of progeny RNA accumulation for viral and subviral pathogens, such as satellite RNAs, in plant hosts. Here, we describe a detailed Northern blot protocol for efficient detection and quantification of viral and satellite RNAs from plant hosts.