Abstract
Nitric oxide formation in ischemia-reperfusion injury can be identified and measured directly using EPR of nitroso-heme complexes comprising NO bound to either Mb or Hb-Fe2+. This article described the successful use of this method to detect and quantify the generation of NO formed independently of nitric oxide synthase in ischemia-reperfusion injury to skeletal muscle. The quantification of nitroso-heme complexes using EPR is recommended in ischemia-reperfusion studies of either skeletal or cardiac muscle that aim to characterize the role of nitric oxide.
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