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Abstract
AbstractThis unit describes methods for the separation and quantification of neurotransmitters, peptides, and second messengers in microdialysis samples by high‐pressure liquid chromatography (HPLC) coupled to either electrochemical detection (EC) or fluorescence detection. The protocols describe commonly used procedures for the detection of catecholamines and indoleamines (HPLC‐EC detection; detection of acetylcholine (HPLC‐EC detection with enzyme reactor), and detection of amino acids (HPLC‐fluorescence detection). Each of these protocols affords detection limits in the low nanomolar range.
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