DETECTION AND QUANTIFICATION OF GROWTH FACTORS IN UMBILICAL CORD MESENCHYMAL STEM CELL-DERIVED EXOSOMES AND UMBILICAL CORD BLOOD-ORIGNATED PLATELET RICH PLASMA

Abstract

Objective: Describing the expression of several growth factors in exosomes released byumbilical cord-derived mesenchymal stem cells (UCMSCs) and platelet rich plasma (PRP)derived from umbilical cord blood (UCB). Methods: 05 UCMSC samples were cultured for therelease of exosomes into conditioned media. 05 UCB-dereived plasma samples were used toprepare PRP. Conditioned media was harvested from UCMSCs at passage four (P4) wasdifferentially centrifuged for exosomes. Isolated exosomes and PRP were then investigated forthe expression of growth factors. The growth factors which are from exosomes and PRP include:hepatocellular growth factor (HGF), platelet-derived growth factor BB (PDGF-BB), vascularendothelial growth factor A (VEGF-A), and fibroblast growth factor 2 (FGF-2). Results: BothUCMSC-derived exosomes and UCB-derived PRP carried growth factors with different amount.The expression of HGF (2176.82 pg), PDGF-BB (1765.82 pg) and VEGF-A (2336.43 pg) is muchgreater than FGF-2 (39.64 pg) and HGF (2176.82 pg) is larger than PDGF-BB (1765.82 pg) inthe UCB-derived PRP. Regarding UCMSC-derived exosomes, HFG (312.62 pg) and PDGF-BB(674.87 pg) were expressed larger than VEGF-A (72.50 pg) and FGF-2 (34.51 pg). Conclusion:Both UCMSC-derived exosomes and UCB-derived PRP contained abundant amount of growthfactors, including HGF, PDGF-BB, VEGF-A, and FGF-2. Expression level of each factor isdifferent and the FGF-2 is the lowest expression in both exosomes and PRP.