Abstract
Rotaviruses (RVs) are the leading cause of diarrhoea in cattle and buffalo calves worldwide. A total of 100 faecal samples were collected from the diarrhoeic cattle and buffalo calves from the organised dairy farms in the Mathura region. Extraction of viral RNA was done in 10% faecal suspension using phenol: chloroform/TRI reagent. The extracted RNA was subjected to RNA-polyacrylamide gel electrophoresis and silver staining. In total, 12 samples were positive for Group A RV, showing 11 segments of RNA genome of the virus in the pattern 4:2:3:2. Based on the migration of segments 10 and 11, all positive samples were characterised as long electropherotypes. Differences in the migration pattern of class I segments was observed. In type 1 pattern, all the four segments (1, 2, 3 and 4) migrated separately. In type 2 pattern, segments 2 and 3 co-migrated. In all the samples, class III segments (7, 8 and 9) moved as a single segment. One sample showed the presence of an additional band between gene segments 5 and 6. Multiple bands were present in two samples. A 12% prevalence of Group A bovine RV was found in the present study in the Mathura region. The samples were positive in the months of August-November and in calves aged below 1 month.
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